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KMID : 0378019620050120067
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New Medical Journal
1962 Volume.5 No. 12 p.67 ~ p.84
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Histopathological Studies on the Effects of Radioactive Phosphorus(P^32) in Albino Rats
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Abstract
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The early and late effects of radioactive phosphorus(P32) were histopathologicaliy studied in albino rats. Twenty four animals were divided into four groups to which various doses of P32 were intrap3ritoneally given for purposes to investigate the early effects of P3´ on the body weight, pripheral blood pictures and other organs. The P32 doses given were; 4.5,ac/g, single, to Group I; 1.0,uz/g, single, to Group II; 2.0,uc/rat, daily for 30 days, to Group 111; and 0.l uc/rat, daily for 30 days to Group IV. The body weight showed a gradual increase in all groups but Group I, which, at first, showed a decrease until 10th day, and then recovered to the initial level on 25th day. The leucocytes count of th-a peripheral blood showed a decrease in Group I between 5th and 15th day, and a slight decrease in Group 11 on 5th day, which were also followed by a gradual recovery. In other groups, no remarkable change was noted. The changes of the erythrocytes count appeared to be similar, showing a maximum reduction on 10th day in ord,-r of Group 1, 11 and 111, while a slight decrease was observed in Group IV. The bone marrow damage was rrost outstanding in Group I on 10th day, while was less severe in Group II, showing the evidence of recovery after 20 days. No remarkable change of the bone marrow was noted in Group III and IV. The spleen revealed the remar-kable damages in Groups I and II, while the other failed to show any notable changes. the recovery changes were distinctly observed after 20 days in Group 11, but scare-ply in Group 1. More or less the similar changes as in the spleen were observed in the lymph nodes. The microscopical examinations of salviary glands disclosed rather distinct and interesting alterations
In all animals which received single large dose of P-32, section showed marked epithelial dyspiastic changes. Nuclei were markedly enlarged with increased staining capacity with clump chromatin pattern and distint nucleoli. There was moderate degree of nuclear pleomorphism with frequent double nucleated cells. L´et mitotic figure was not frequent. In parallel to the cellular atypism, there was marked disturbance of (rranoid pattern. Acinar structure is completely abolished and cells were arranged in random fashion. At the first glance, the picture was just like carcinoma but active mitosis and invasive nature was lacking Such changes were more distinct in the early stage and much less in late stage.
On the other hand, thirty seven animals were divided into four groups to which various doses of P32 were in t raper itoneally given for purposes to investigate the late-especially its carcinogf:Ice-e_`f~,c s of P32 in animals. The P32 doses given were; 4.5,uc/g, single; 4.5,uc/g plus cortisone acetate 10mg, sin ;le; monthly for 5 to 7 months; and 2,uc/rat, daily for 60 to 340 days.
Osteogenic sarcoma occurred in one case which received a single dose of 4.5,uc/g with a la:~ i~ i):riod of 260 days and in four cases which received the repeated doses of l uc/g with an average latent preiod of 170 days. The predilection site of the tumor was the femur, and the metastases to the lungs were observed in two animals. The histopathological changes of the bone marrow, spleen, liver, lymph nodes, kidney and salivary gland were also investigated. The other organs, however, showed no specific histopathological changes which could be attributed to the effects of irradiation.
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